【摘要】 目的研究人参总皂苷(TSPG)对脐血造血细胞(UCB)红细胞生成素受体(EPOR)表达的影响。方法以25、50、75和100mg/LTSPG刺激脐血单个核细胞24h,采用流式细胞仪检测细胞膜EPOR表达的变化;细胞Elisa实验检测细胞膜EPOR表达量的改变;免疫细胞化学观察细胞EPOR阳性率的变化。结果流式细胞仪检测显示50mg/LTSPG均能提高脐血细胞膜表面EPOR的表达;细胞Elisa实验结果显示50mg/LTSPG能够提高脐血造血细胞膜表面EPOR的表达量;免疫细胞化学观察显示TSPG50mg/L组单个核细胞的EPOR表达阳性率较对照组显著增高(P<0.01)。结论适当剂量(50mg/L)的TSPG可增强脐血单个核细胞膜表面EPOR的表达。更多还原

【Abstract】 Objective To investigate the effect of total saponins of panax ginseng (TSPG) on expression of EpoR in hematopoietic cells from umbilical cord blood (UCB). Methods After mononuclear cells (MNCs) from UCB induced by 25,50,75 and 100 mg/L TSPG for 24 h respectively,the quantity of EPOR expression on MNCs was detected by using flow cytometry (FCM) and cell Elisa. EPOR protein express positive ratio of MNCs was investigated by immunocytechemistry. Results FCM and cell Elisa shows 50 mg/L TSPG promotes to raise the expression quantity of EPOR on MNCs Compared with control group,immunocytechemistry shows EPOR protein positive ratio on MNCs is markedly increased with the induction of TSPG 50 mg/L. Conclusion Some certain concentrate of TSPG (50mg/L) can enhance the expression of EPOR on MNCs from UCB.更多还原