【摘要】 目的进一步观察移植后内皮细胞(EC)抗切应力和抗钙化能力以及肌成纤维细胞迁移和自身修复能力。方法新鲜牛心包片经脱细胞、鞣制、改性后备用;A、B组试片上依序种植宿主肌成纤维细胞和EC,C组试片上不种植细胞,随后将3组试片分别移植于猪的腹主动脉壁上;2个月后对A组试片,12个月后对B、C组试片进行厚度、钙含量、扫描电镜及组织学的检测。结果A、B组试片钙含量显著低于C组(P<0.05),但显著高于新鲜牛心包(P<0.05);A、B组试片钙含量差异无显著性(P>0.05);A、B组试片显著厚于C组试片(P<0.05),A、B组试片的厚度差异无显著性(P>0.05);B组试片80%～90%的表面覆盖EC,肌成纤维细胞已迁移至表层下支架的3/4;C组试片表面无细胞,肌成纤维细胞已迁移至表层下支架的1/4～1/3,大部分胶原纤维间隙消失。结论人工EC化使生物材料抗钙化能力增强;种植肌成纤维细胞使生物材料具有自身修复能力。但增厚的瓣叶是否影响其功能,尚有待进一步研究。更多还原

【Abstract】 Objective To further analyze the ability of resisting the shear-stress and anti-calcification of endothelial cells (EC),and further analyze the ability of migrating and self-repair of myofibroblasts. Methods The fresh bovine pericardia were acellularized,tanned and modified; Autologous myofibroblasts and ECs were seeded on the patches of Group A and B sequentially; Group C was unseeded control group. Then the patches of all groups were implanted into the porcine abdominal aortic wall separately; The retrieved specimens of Group A were evaluated by the studies of thickness,calcium content,scanning electron microscopy (SEM) and histological examination 2 months later,Group B and C were evaluated 12 months later. Results The specimen calcium of Group A and B was less than that of Group C significantly (P <0.05),but more than that of fresh pericardial group significantly (P <0.05); And the specimen calcium of Group A and B was not significantly different (P >0.05); The specimen thickness of Group A and B was thicker than that of Group C significantly (P <0.05),but it was not significantly different in Group A and B (P >0.05); In Group B,ECs covered 80%~90% of the specimen surface,myofibroblasts proliferated and immigrated into 3/4 thickness of the matrix; In Group C,no cells were found on the surface of specimens,myofibroblasts proliferated and immigrated into 1/4~1/3 thickness of the matrix,and most of interfiber fissures disappeared. Conclusions Artificial endothelialization of the biological material may improve the ability of preventing bioprosthetic calcification; The seeded myofibroblasts may offer the ability of self-repair of bioprostheses. But will the valve leaflets with increased thickness interfere with the function? It’s still to be determined.更多还原