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克林霉素磷酸酯脂质体的制备

The preparation of clindamycin phosphate liposome

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【作者】 何剪太周伟华张阳德郭讯枝曾昭武李珍发刘星言谭斐赵劲风潘一峰

【Author】 HE Jian-tai1, ZHOU Wei-hua2, ZHANG Yang-de1, GUO Xun-zhi2, ZENG Zhao-wu2, LI Zhen-fa1, LIU Xing-yan2, TAN Fei2, ZHAO Jin-feng2, PAN Yi-feng2 (1.Xiangya Hospital of Centre South University, National Hepatobiliary and Enteric Surgery Research Center, the Ministry of Health, Changsha, Hunan 410008, P.R.China; 2.Institute of Biomedical Engineering of Centre South University, Changsha, Hunan 410008, P.R.China; 3.Yichun University of Jiangxi, Yichun, Jiangxi 336000, P.R.China)

【机构】 中南大学湘雅医院卫生部肝胆肠外科研究中心中南大学生物医学工程研究院中南大学生物医学工程研究院 湖南长沙410008湖南长沙410008江西宜春学院江西宜春336000湖南长沙410008

【摘要】 目的制备克林霉素磷酸酯脂质体并测定包封率及粒径。方法采用蒸发超声法制备克林霉素磷酸酯脂质体,高效液相色谱法检测克林霉素磷酸酯包封率,用激光粒度仪测定其平均粒径。结果运用正交设计方法,优化脂质体的制备工艺和处方为:磷脂:胆固醇为5∶1,超声时间为6min,蒸发温度为35℃,磷脂:克林霉素为5∶3,磷脂:海藻糖为2∶1。克林霉素磷酸酯在5.0~50μg/mL范围内线性关系良好。3组克林霉素磷酸酯脂质体包封率分别为52.26%、50.13%和53.75%。结论克林霉素磷酸酯脂质体制备工艺可行,质量控制方法简单、准确。

【Abstract】 [Objective] To prepare clindamycin phosphate liposome, and to determine the entrapment efficiency and particle diameter of it. [Methods] Clindamycin phosphate liposome was prepared by evaporating and ultrasound method, to determine the entrapment efficiency of Hydroquinone liposome by HPLC and the average particle diameter by laser particle size analyser. [Results] Using orthogonal design, the preparation technology of liposome and the prescription was: phospholipid: cholesterin 5∶1, ultrasound time was 6min, evaporating temperature was 35℃; phospholipids: Clindamycin: 5∶3; phospholipids: trehalose: 2∶1. Clindamycin Phosphate had a good linear relation in a range of 5.0~50.0 μg/mL. The entrapment efficiency of clindamycin phosphate liposome in three groups reached 52.26%, 50.13% and 53.75% respectively. [Conclusion] The technique of preparing clindamycin phosphate liposome is feasible and the method of quality control is simple and accurate.

【基金】 国家863资助项目(No:2007AA022008)
  • 【文献出处】 中国现代医学杂志 ,China Journal of Modern Medicine , 编辑部邮箱 ,2008年01期
  • 【分类号】TQ460
  • 【被引频次】8
  • 【下载频次】318
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