【摘要】 目的制备克林霉素磷酸酯脂质体并测定包封率及粒径。方法采用蒸发超声法制备克林霉素磷酸酯脂质体,高效液相色谱法检测克林霉素磷酸酯包封率,用激光粒度仪测定其平均粒径。结果运用正交设计方法,优化脂质体的制备工艺和处方为:磷脂:胆固醇为5∶1,超声时间为6min,蒸发温度为35℃,磷脂:克林霉素为5∶3,磷脂:海藻糖为2∶1。克林霉素磷酸酯在5.0～50μg/mL范围内线性关系良好。3组克林霉素磷酸酯脂质体包封率分别为52.26%、50.13%和53.75%。结论克林霉素磷酸酯脂质体制备工艺可行,质量控制方法简单、准确。更多还原

【Abstract】 [Objective] To prepare clindamycin phosphate liposome, and to determine the entrapment efficiency and particle diameter of it. [Methods] Clindamycin phosphate liposome was prepared by evaporating and ultrasound method, to determine the entrapment efficiency of Hydroquinone liposome by HPLC and the average particle diameter by laser particle size analyser. [Results] Using orthogonal design, the preparation technology of liposome and the prescription was: phospholipid: cholesterin 5∶1, ultrasound time was 6min, evaporating temperature was 35℃; phospholipids: Clindamycin: 5∶3; phospholipids: trehalose: 2∶1. Clindamycin Phosphate had a good linear relation in a range of 5.0~50.0 μg/mL. The entrapment efficiency of clindamycin phosphate liposome in three groups reached 52.26%, 50.13% and 53.75% respectively. [Conclusion] The technique of preparing clindamycin phosphate liposome is feasible and the method of quality control is simple and accurate.更多还原