【摘要】 目的观察淫羊藿苷和按红芪减方由淫羊藿苷、红芪多糖、地龙水提液组成的混药对大鼠周围神经再生的促进作用。方法制备浓度为0.005g/mL的淫羊藿苷溶液和浓度约等于1g(原药材)/mL的由淫羊藿苷、红芪多糖、地龙水提液组成的混药。取体重为(200±10)g的雄性SD大鼠20只,随机分为4组(n=5),分别为假手术组(A组)、模型组(B组)、淫羊藿苷组(C组)和混药组(D组)。A组仅暴露左侧坐骨神经后缝合皮肤,不钳夹神经;B、C和D组以钳夹左侧坐骨神经的方式制备大鼠周围神经损伤模型;右侧不作任何处理。于次日开始每日定时灌胃给药,A组和B组给予生理盐水;C组给予淫羊藿苷溶液,D组给予混药,均为2mL/d。给药后观察大鼠一般情况,给药后第21天检测神经功能指数、坐骨神经传导速度和有髓坐骨神经纤维形态及数量。结果术后各组大鼠存活至实验完成,一般状况良好。第21天,A、B、C及D组大鼠体重分别为(366.9±14.0)、(370.1±16.3)、(373.3±19.6)及(374.0±11.4)g,各组间差异无统计学意义(P>0.05)。坐骨神经功能指数测定A组与D组、B组与C组间差异无统计学意义(P>0.05),余各组间差异有统计学意义(P<0.05);胫神经功能指数,A组与B、C、D组间差异有统计学意义(P<0.05),B组与C、D组间差异无统计学意义(P>0.05);腓总神经功能指数,A组与C、D组间、B组与C、D组间差异无统计学意义(P>0.05)。A、B、C和D组坐骨神经传导速度分别为(45.0±2.9)、(8.0±2.6)、(13.4±6.8)及(19.6±9.3)m/s,B组与C组间差异无统计学意义(P>0.05),A组与B、C、D组间,B组与D组间差异有统计学意义(P<0.05)。锇酸染色观察B、C、D组的再生有髓坐骨神经纤维较A组细;B、C、D组再生有髓坐骨神经纤维数分别为A组的93.3%±35.6%、90.6%±37.1%和115.4%±40.6%,各组间差异均无统计学意义(P>0.05)。结论淫羊藿苷和混药具有良好的安全性,混药促周围神经再生作用较淫羊藿苷显著,提示应整体研究红芪减方,以期发现有效部位或多个化合物以固定配比组成的有效成分群。更多还原

【Abstract】 Objective To investigate the effects of icariin and mixed prescription of icariin,radix hedysari polysaccharide,and liquid extracted from earthworm on peripheral nerve regeneration.Methods Twenty male SD rats weighing(200 ± 10) g were selected and randomized into four groups(n=5 per group):sham operated group(group A),model group(group B),icariin group(group C),and mixed liquid group(group D).In group A,the left sciatic nerves of the rats were only exposed,and treated at xed time from the following day with the NS(2 mL/d).In groups B,C,D,the models were made by clamping sciatic nerve and treated with NS,icariin and mixed liquid,respectively(2 mL/d).The general state of animals was observed after the treatment daily.The nerve function index,motor nerve conductive velocity and the morphous and number of myelinated sciatic nerve bers were measured at 21 days.Results Animals in various groups were all in good state.After 21 days,the weights of rats in groups A,B,C and D were(366.9 ± 14.0),(370.1 ± 16.3),(373.3 ± 19.6) and(374.0 ± 11.4) g,respectively,and there was no signi cant di erence among these groups(P > 0.05).For sciatic function index,there was no signi cant di erence between group A and group D(P > 0.05),between group B and group C(P > 0.05),while there was signi cant di erence between group B and group D(P < 0.05).For tibial function index,there was signi cant di erence between group A and groups B,C,D(P < 0.05),there was no signi cant di erence between group B and groups C,D(P > 0.05).For peroneal function index,there was no signi cant di erence between group A and groups C,D(P > 0.05),between group B and groups C,D(P > 0.05).The sciatic motor nerve conductive velocities of group A,B,C and D were(45.0 ± 2.9),(8.0 ± 2.6),(13.4 ± 6.8),and(19.6 ± 9.3) m/s,respectively,there was no signi cant di erence between group B and group C(P > 0.05),and there was signi cant di erence between group A and groups B,C,D and between group B and group D(P < 0.05).The size of individual myelinated sciatic nerve bers of regenerated nerves in groups B,C,and D was signi cantly smaller than that in group A.Comparing with group A,the number of myelinated sciatic nerve bers in groups B,C,and D was 93.3% ± 35.6%,90.6% ± 37.1%,and 115.4% ± 40.6%,respectively,but there was no signi cant di erence among four groups(P > 0.05).Conclusion Icariin and mixed prescription are safe.The improving peripheral nerve regeneration e ect of mixed prescription is more obvious than that of icariin,indicating the comprehensive study of modi ed formula radix hedysari is necessary to nd the e ective part or mixture of e ective compounds with xed percentage.更多还原