【摘要】 以薄荷茎段为外植体,研究了不同植物激素对薄荷愈伤组织的诱导及其增殖、不定芽分化和诱导生根的影响。结果表明,愈伤诱导最适培养基为MS+6-BA1.5mg/L+2,4-D0.5mg/L+NAA0.5mg/L,其愈伤组织生长良好;最佳愈伤增殖培养基为MS+NAA1.0mg/L或MS+IAA2.0mg/L,其褐化率均较低;最佳诱导分化培养基为MS+6-BA2.0mg/L+NAA0.1mg/L,分化率达77%;生根培养基为1/2MS+NAA2.0mg/L,且根较为粗壮。更多还原

【Abstract】 The stem segments of Mentha canadensis were used as explants to study the effect of plant regulators on callus induction,callus multiplication,adventitious buds differentiation and rootage.The results showed that MS+1.5mg/L 6-BA+0.5mg/L 2,4-D+0.5mg/L NAA was the most appropriate media for callus induction for callus growth.MS+1.00mg/L NAA or MS+2.00mg/L IAA was the most appropriate media for callus multiplication with low browning rate.MS+2.0mg/L 6-BA+0.1mg/L NAA was the most appropriate media for differentiation of adventitious buds,with the efficiency of 77%.1/2MS+2.0mg/L NAA was the most appropriate media for rooting.更多还原