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大豆疫霉根腐病菌毒素处理抗感不同大豆品种后苯丙氨酸解氨酶活性的变化

Change of Phenylalanine Ammonia-lyase (PAL) Activities among Different Resistance Soybean Varieties Treated with Pathotoxin from Phytophthora sojae

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【作者】 张淑珍徐鹏飞吴俊江王萍陈维元陈晨王金生靳立梅于安亮张大勇李文滨

【Author】 Zhang Shuzhen1,Xu Pengfei1,Wu Junjiang2,Wang Ping3,Chen Weiyuan4,Chen Chen1, Wang Jinsheng1,Jin Limei1,Yu Anliang1,Zhang Dayong1,Li Wenbin1(1Soybean Institute,Northeast Agricultural University,Harbin 150086,Heilongjiang; 2Soybean Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,Heilongjiang; 3Ocean College,Huaihai Institute of Technology,Lianyungang 222005,Jiangsu; 4Suihua Institute of Agricultural Sciences,Heilongjiang Academy of Agricultural Sciences,Suihua 152052,Heilongjiang,China)

【机构】 东北农业大学大豆研究所黑龙江省农业科学院大豆研究所淮海工学院海洋学院黑龙江省农业科学院绥化农科所东北农业大学大豆研究所 150030黑龙江哈尔滨150030

【摘要】 对大豆疫霉根腐病菌毒素胁迫下抗感不同大豆品种根、茎、叶中苯丙氨酸解氨酶(PAL)活性的变化规律进行了初步研究,结果表明:适宜浓度的毒素(稀释100倍,浓度为0.0897mg/mL)处理抗病大豆品种的根、茎和叶中苯丙氨酸解氨酶活性在病程的大部分阶段与对照相比都升高,而感病品种在整个病程中虽然在某些阶段较对照有一定的提高,但幅度不大,在病程其他阶段苯丙氨酸解氨酶下降幅度远大于升高幅度。而浓度相对较高的毒素(稀释50倍,浓度为0.1794mg/mL)处理后抗感品种根、茎和叶中苯丙氨酸解氨酶活性的变化较稀释100倍浓度毒素幅度小。

【Abstract】 Change of phenylalanine ammonia-lyase (PAL) activities among different resistance soybean varieties treated with pathotoxin from Phytophthora sojae was preliminarily studied in this paper.The results showed that the optimum concentration of pathotoxin (0.0897mg/mL) led to higher PAL activities in resistant varieties than those of control variety during most stages of disease processing.Contrarily,susceptible varieties were possessed of medium high levels of PAL activities with a rapid declination only during few limited stages of the disease processing.However,treatment with high concentration of pathotoxin (0.1794mg/mL) caused low levels of PAL activities in both resistance varieties and susceptible ones compared with treatment of the optimum concentration.

【基金】 国家自然科学基金(30400285,30671317);中国博士后基金(20060400835);黑龙江省新世纪优秀人才培养计划(NCET-06-007);黑龙江省高校骨干教师项目(1151G007);黑龙江省农科院博士后基金(LRB06-010);博士后落户黑龙江科研启动基金(LBH-Q05032);黑龙江省青年基金(QC06C012);农业部寒地重点开放实验室开放课题
  • 【分类号】S435.651
  • 【被引频次】40
  • 【下载频次】856
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