【摘要】 目的:建立硝基呋喃类药物(呋喃唑酮、呋喃它酮、呋喃西林和呋喃妥因)代谢物的液相色谱-串联质谱分析方法。方法:硝基呋喃类代谢物经过二硝基苯甲醛衍生化,用乙酸乙酯提取后经液相色谱分离,电喷雾串联质谱法进行检测。结果:4种硝基呋喃代谢物的定量线性范围均为1~50 ng/ml,线性关系良好(r>0.99),水产品中的定量检测限均为0.5μg/kg,回收率在88.0%~94.3%范围内,相对标准偏差在10.4%以下。结论:本法专属性强,灵敏度和准确度高,可作为分析水产品中硝基呋喃类代谢物残留量的有效手段。更多还原

【Abstract】 Objective:To develop a LC-MS/MS method for the simultaneous determination of the metabolites of four nitrofuran antibacterial agents,furazolidone,furaltadone,nitrofurazone and nitrofurantoin in aquatic products.Methods:Samples were derivatised with 2-nitrobenzaldehyde and extracted by ethyl acetate.The analytes were separated and detected by tandem mass spectrometry with electrospray ionization source.Results:The limits of quantification for four nitrofuran metabolites in samples were 0.5 μg/kg.The matrix-matched calibration curve showed good linearity(r2>0.99) in the range of 1~50 ng/ml,The mean recovery was between 88.0% and 94.3% and the relative standard derivations less than 10.4%.Conclusion:This method is specific,sensitive and accurate.It is reliable to determine the nitrofuran metabolites in aquatic products.更多还原