【摘要】 从进口日本豇豆中随机挑选种子在隔离温室内种植。出苗后,发现有典型花叶症状的植株。对该病株进行电镜观测,发现有线条形病毒粒体,大小约为750nm×13nm。设计特异性引物(BL-5/BL-6),对该样品进行黑眼豇豆花叶病毒的反转录(RT)PCR分子检测。电泳检测表明,扩增出一条特异性的目标条带,大小为338bp。对该样品的RT-PCR产物进行测序,结果表明该序列与黑眼豇豆花叶病毒(GENEBANK登录号:AY575773)的序列有4个碱基差异,同源性为98.8%。鉴定该病毒为黑眼豇豆花叶病毒(Blackeye cowpea mosaicvirus)。更多还原

【Abstract】 The cowpea seeds imported from Japan were selected and sowed in isolated greenhouse.After seedling,some plants were showing typical mosaic symptom.Electron microscopic observation by dip method reveals the particle of the virus as a flexuous rod measuring 750nm×13nm.A pair of specific primers(BL-5/BL-6)based on blackeye cowpea mosaic virus was designed to detect the virus by reverse transcript(RT)PCR,electrophoresis results showed a specific band measuring 338bp was amplified.The product of RT-PCR was further sequenced and showed there were only 4 bases difference between the product and BlCMV(AY575773),the homology is 98.8%.Therefore,Blackeye cowpea mosaic virus(BlCMV)was screened and identified from the cowpea.更多还原