【摘要】 目的观察硫辛酸(LA)对氧化低密度脂蛋白(ox-LDL)诱导的大鼠胸主动脉平滑肌细胞(VSMC)内甘油三脂(TG)、总胆固醇(TC)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX)活性的影响。方法选取SPF级、雄性SD大鼠20只,安乐死后分离胸主动脉血管中膜,将获得的VSMC分为5组:空白对照组、ox-LDL组、ox-LDL+LA(20、80、100mg/L)组。空白对照组不加任何药物,各实验组以ox-LDL为泡沫细胞诱导剂,加入不同浓度的LA,分别测定VSMC内TG、TC、SOD和GSH-PX的活性。结果ox-LDL组TG、TC、SOD和GSH-PX值与空白对照组比较差异有统计学意义(P<0.05);LA各浓度干预组(20、80和100mg/L)TG、TC、SOD和GSH-PX值与ox-LDL组比较差异有统计学意义(P<0.05),各LA剂量组之间比较差异有统计学意义(P<0.05)。结论LA可抑制ox-LDL诱导VSMC的脂质堆积,抑制泡沫细胞的形成,这可能与其抗氧化活性有关。更多还原

【Abstract】 Objective To investigate the effects of lipoic acid on triglyeride(TG),total cholesterol(TC),and the activities of superoxide dismutase(SOD) and glutathione peroxidase (GSH-PX) in cultured vascular smooth muscle cells(VSMC) induced by ox-LDL. Methods Twenty special pathgon free and male SD rats were sacrificed and isolated their thoracic aorta VSMC. VSMC was divided into five groups: control,ox-LDL,ox-LDL with different lipoic acid (20,80,100 mg/L) and the control group donot were gave any drugs,and four experiment groups were exposed to ox-LDL and then incubated with different concentrations of lipoic acid respectively. The levels of TG and TC were measured and the activities of SOD and GSH-PX were detected in VSMC. Results It were found that the levels of TG,TC in ox-LDL group was significantly higher than those in the control group(P<0.05),the activities of SOD and GSH-PX were much lower than those in the control group(P<0.05). Lipoic acid could significantly lower the levels of TG,TC(P<0.05) and enhance the levels of SOD and GSH-PX(P<0.05) in VSMC induced by ox-LDL. Conclusion Lipoic acid can inhibit lipid accumulation in VSMC induced by ox-LDL. The mechanisms may be associated with the increase of self-anti-oxidation ability of lipoic acid.更多还原