【摘要】 目的研究大鼠骨髓间充质干细胞的分离培养方法,建立大鼠肝脏缺血-再灌注损伤动物模型,观察骨髓间充质干细胞对大鼠肝脏缺血-再灌注损伤的修复作用。方法体外培养大鼠骨髓间充质干细胞,建立大鼠肝脏缺血-再灌注损伤的模型。动物模型制备后,将30只大鼠随机分为实验组(即尾静脉注入骨髓间充质干细胞0.5 ml,约106个)10只,阴性对照组(尾静脉注入生理盐水0.5 ml)10只。以正常大鼠作为空白对照组10只。结果各组大鼠于1周,2周,4周进行采血,取脏器进行各项指标检测。肝脏缺血-再灌注损伤大鼠在第1周实验组和阴性对照组的丙氨酸转移酶(ALT)、门冬氨酸转移酶(AST)和丙二醛(MDA)均明显高于空白对照组,差异显著,P<0.05。第2周时,实验组大鼠ALT、AST和MDA与阴性对照组比较,明显低于阴性对照组P<0.05,但SOD明显高于阴性对照组P<0.05。第4周时,实验组大鼠ALT、AST和MDA与阴性对照组比较,明显低于阴性对照组P<0.05。但实验组与空白对照组MDA和SOD有差异P<0.05。结论大鼠骨髓间充质干细胞对大鼠肝脏缺血-再灌注损伤的修复可起促进作用。更多还原

【Abstract】 Objective To study the method of isolated culture of bone marrow mesenchymal stem cells(MSCs),to establish the rat model of hepatic ischemia-reperfusion,and observe the effect of MSCs to the hepatic ischemia-reperfusion injury.Methods MSCs of rats were cultured in vitro,rats were divided randomly into the treated group and the negative control group.The treated group were injected MSCs 0.5 ml,the control group were injected normal saline 0.5 ml.The normal rats were blank control group.Results All animals blood were collected after 1 and 2、4weeks to observe the serum activities of ALT,AST,MDA and SOD.At first week,the serum activities of ALT、AST and MDA in treated group and negative control group were higher than the blank control group(P<0.05).There’s no much difference between the treated group and negative control group(P>0.05).At second week,the serum activities of ALT,AST and MDA in treated group were lower than negative control group(P<0.05),but the serum activities of SOD in treated group was higher than negative control group(P<0.05).At four week,the serum activities of ALT,AST and MDA in treated group were lower than negative control group(P<0.05),and ALT,AST have no difference between blank control group(P>0.05),but MDA,SOD have much difference(P<0.05).Conclusion MSCs can promote cells proliferation of the hepatic ischemia reperfusion injury.更多还原