【摘要】 斑点金标免疫渗滤新技术(简称金标法)检测家畜血吸虫病抗体,在本省进行了特异性、敏感性、符合率等试验。金标法能检出人工感染犬血吸虫14d后的血吸虫病抗体,检出率为100%,可用于血吸虫病早期诊断。粪孵100头份血吸虫病阳性的牛血纸,用金标法检测全部为阳性,阳性符合率为100%;在非疫区的2个县分别采牛血纸100份,用金标法进行检测,未查出血吸虫病阳性牛,阴性符合率为100%。在金标法检测4000份牛血纸,检出阳性牛为129头,再对这129头牛采粪孵化,有血吸虫毛蚴的牛128头,金标法与病原学检查法阳性符合率为99.2%(99%~100%)。在本省54个血防疫区县推广应用,取得了显著的社会经济效益。更多还原

【Abstract】 A new dot immunogold filtration assay(DIGFA)technology to detect circulating anti-schistosome antibodies in livestock was carried out for evaluation of its sensitivity,specificity,and consistency.It could detect the circulating anti-schistosome antibodies in blood paper lixivium from cattle 14 days post infection with schistosome cercaria while it could detect cerearia faeces hatching miracidium(FHM)for 42-56 days.DIGFA’s detection rate is 100%,and suitable for the early diagnosis of animal’s schistosomiasis.In 100 blood paper positive samples of cattle infected by schistosomiasis diagnosed with FHM method,the positive rate of DIGFA detection was 100%,which indicated that the coincidence rate is 100% between DIGFA and FHM methods.In the same study,out of 4 000 blood paper samples of cattle in epidemic areas of this disease 129 schistosomiasis positives as examined with DIGFA.The follow-up study showed that 128 infected cattle were positive as detected with FHM method,the consistent rates bewteen DIGFA and FHM is 99.2%.DIGFA method has been used in 54 counties of epidemic areas of infectious schistosomiasis,and yielded obvious socioeconomic benefits.更多还原