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蒙古绵羊β-防御素的纯化及其抗菌活性研究

Isolation and characteristic of Mongolia Ovis aries β-defensin

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【Author】 LI Yong-lan1,2,SHAO Yan-hong1,QIU Guang-liang2,WANG Xiu-mei1,CAO Gui-fang1(1.College of Animal Science and Technology,Inner Mongolia Agricultural University,Huhhot 010018,China;2.Centre of Biotechnology,Inner Mongolia Normal University,Huhhot 010022,China)

【机构】内蒙古农业大学动物科学与医学学院；内蒙古师范大学生物工程研究中心；内蒙古农业大学动物科学与医学学院内蒙古呼和浩特010018内蒙古师范大学生物工程研究中心；内蒙古呼和浩特010022；内蒙古呼和浩特010018；

【摘要】采用高盐低pH缓冲液进行粗提,粗提液经阳离子交换层析-RP HPLC-Sephadex G25凝胶过滤-C18RP-HPLC纯化,得到纯化多肽。尿素-Tricine-SDS PAGE测定其分子量为3.8kDa,215nm具最大吸收。对Ecoli、S.aureus、B.proteus和B.subtilis有强的抗菌活性。更多还原

【Abstract】 To isolate and purify β-defensin(SBD)from the small intestine of Mongolia Ovis aries,acid soluble extract of Mongolia Ovis aries small intestine was obtained.Then it was further purified by cation exchange chromatography,RP-HPLC,G-25 gel filtration,and RP-HPLC.The antibacterial activities of the extracts from every step were analyzed by agarose radial diffusion assay.The molecular weight of the purified SBD was determined to be 3.8 kDa by Tricine-SDS-PAGE.SBD-2 from Mongolia Ovis aries small intestine was able to effectively kill E.coli,S.aureus,B.subtilis and Bacillus proteus.SBD-2 has an important part in innate immunity in Mongolia Ovis aries.更多还原

【关键词】蒙古绵羊；分离纯化； β-防御素；
【Key words】 Mongolia Ovis aries； isolation and purification； β-defensin-2；

【基金】国家自然科学基金资助项目(30460093)

【文献出处】中国兽医杂志 ,Chinese Journal of Veterinary Medicine , 编辑部邮箱 ,2008年02期

【分类号】S852.4
【被引频次】5
【下载频次】130

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