【摘要】 本研究应用B超引导阴道穿刺法,对40头尼里-拉菲和摩拉水牛连续3次采卵,采卵间隔为3d,共采卵120头次。共穿刺卵泡935个,采集卵母细胞643枚,可用卵母细胞为456枚,卵母细胞回收率为68.77%(643/935),卵母细胞可用率为70.92%(456/643),头均可用卵母细胞为(3.80±0.23)个。第1、2、3次采卵头均回收可用卵母细胞数分别为(3.82±0.50)、(3.82±0.35)、(4.05±0.43)枚,差异不显著(P>0.05)。收集的卵母细胞经体外成熟、体外受精和受精卵与颗粒细胞单层体外共培养,受精后48h分裂率为56.36%(257/456),囊胚率为20.39%(93/456)。2个品种水牛的头均可采卵泡数、头均回收卵母细胞数和囊胚率均无显著差异。这表明,在B超引导下进行连续活体采卵,并利用卵母细胞进行体外受精生产胚胎是可行的。更多还原

【Abstract】 Transvaginal ultrasound-guided ovum pick-up(OPU)was performed on 40 Nili-Ravi and Murrah buffaloes for consecutive 3 times each,at an interval of 3 days.A total of 935 follicles were aspirated,643 oocytes were retrieved,while 456 oocytes of them were usable.The recovery rate was 68.77%(643/935),and the percentage of usable oocytes was 70.92%(456/643),an average of 3.80±0.23 oocytes were usable for per buffalo.The number of usable oocytes for the first,second and third OPU was 3.82±0.50,3.82±0.35,4.05±0.43,respectively,the difference among them was not significant(P>0.05).After in-vitro maturation(IVM),in-vitro fertilization(IVF)and co-incubation with single layer of granular cells,the cleavage rate was 56.36%(257/456),blastocyst formation rate was 20.39%(93/456).There’s no significant difference between the two breeds on aspirated follicles,recovery rate of oocytes and blastocyst formation rate of per buffalo.The results proved the feasibility of in-vitro embryo production of oocytes obtained by transvaginal ultrasound-guided consecutive OPU.更多还原