【摘要】 为了解AA肉种鸡群中免疫抑制性病毒的存在状况及其对NDV疫苗免疫效果和对商品鸡生长的影响,采用多重PCR、RT-PCR和血清学方法对山东某规模化大型AA父母代肉种鸡场的28日龄、180日龄左右种鸡及其所生产的1日龄、30日龄左右商品鸡进行IBDV、MDV、ALV、REV、CIAV及REOV感染状况的调查,并同时对鸡群NDV免疫后抗体水平进行跟踪监测。结果表明种鸡和商品鸡均存在CIAV的感染,并不同程度地存在CIAV与MDV、IBDV等的共感染;发病鸡群NDV抗体水平明显偏低的原因是由于鸡群感染免疫抑制性病毒所致;种鸡群感染CIAV并垂直传播给商品鸡,间接引起商品鸡发生免疫抑制性病毒的共感染,导致商品鸡群30日龄左右生长受阻、免疫能力降低、疾病爆发。更多还原

【Abstract】 In order to investigate the infections of immunosuppressive viruses in AA broiler chicken flocks and further study how the viruses influence the function of NDV vaccines and the growth of AA broiler chicken flocks.We select the chickens suspected to be infected by immunosuppressive viruses from one large-scale AA broiler breeder farm and their commercial chicken farms in Shandong province.DNA and RNA were extracted for the multiplex PCR to detect CIAV,MDV,REV,ALV and the RT-PCR to detect IBDV.Also antibody to REOV and NDV were tested.The results showed that CIAV existed in the investigated chicken flocks and coinfections were serious.The infections of immunosuppressive viruses were the main causation for descent or failure of the function of the NDV vaccines.The breeder flocks were infected by CIAV and then the 1d chicken flocks were infected vertically by CIAV which made the death rate of 30 d commercial chicken flocks increasing.更多还原