【摘要】 目的:建立一套从香菇子实体浓缩液中快速高效分离纯化香菇多糖的工艺.方法:采取超滤、乙醇沉淀、DEAE-纤维素柱层析、SephadexG-200凝胶柱层析、聚丙烯酰胺凝胶连续电泳等一系列分离纯化检测方法.结果:通过上述方法,从香菇浓缩液中纯化的LNTⅠb的纯度为95.55%;证实LNTⅠb为糖蛋白.更多还原

【Abstract】 Objective: To establish a efficient craft of isolation and purification lentinan.Methods: Using series of craft,including ultrafiltration,precipitation with ethylalcohol,DEAE-acaellulose column chromatographi,SephadexG-200 column chromatographiy and so on.Conclusion: The purificating rate of LNTⅠb was 95.55%,and it is proved that LNTⅠb is a kind of glycoprotein.更多还原