【摘要】 目的:基于CCD的荧光分子断层成像(FMT)近年来取得了快速发展,非接触式荧光分子断层成像技术(FMT)需要获取成像对象的三维表面轮廓,用于荧光团浓度重建的前向模型。方法:提出一种在白光条件下基于反投影法的三维轮廓提取方法,在获取360°的CCD照片后,滤波反投影重建断层,经过相应的图像平滑处理,得到轮廓边界,校准后重建出表面轮廓。结果:仿体实验和小动物实验结果表明,该方法具有毫米量级的重建精度。结论:该方法能够很好地重建躯干部分表面三维轮廓,较其他表面轮廓提取方法简单、实用,而且能够顺利地结合到荧光成像的工作流程中去,不需要单独成像,保证了整个成像过程的连续性。更多还原

【Abstract】 Objective: The CCD-based non-contact fluorescencemolecular tomography has got a rapid progress in recent years.This imaging modality needs to acquire 3D-surface of the animal imaged,which is used as the forward model in the fluorescence concentration reconstruction.Methods: We proposed a new 3D-surface reconstruction method based on the back-projection method under the white light condition.After taking the surface pictures by CCD,the slice images were reconstructed using filter back-projection method,then the edge curve of each slice was extracted,and based on the data of the edges and calibration operation,we reconstructed the 3D-surface of the animal imaged.Results: The results of both phantom simulation and animal experiment showed that the method properly reconstructed the 3D-surface of trunk of small animals.Conclusions: Compared with other methods,this method is simple and fast.Furthermore,the proposed method enables the surface imaging to be inserted into the working process of the whole FMT system without interruption.更多还原