【摘要】 从神农架国家自然保护区土样中分离筛选到1株抗烟草花叶病毒的放线菌HNS2-2。根据培养性状、扫描电镜观察、生理生化测定以及16S rRNA序列分析,初步判定该菌株为链霉菌属中的草绿色链霉菌(Streptomyces herbaricolor)。HNS2-2菌株培养滤液与烟草花叶病毒混合后接种在枯斑寄主曼陀罗和系统侵染寄主普通烟K326上,对两寄主的枯斑抑制率分别为92.62%和61.88%;接种病毒前、后施用HNS2-2菌株培养滤液对两寄主的枯斑抑制率分别为83.78%、67.26%和54.06%、42.37%。更多还原

【Abstract】 A Streptomyces strain,designated as HNS2-2,were isolated from a soil sample from Shennongjia nature preservation zone and proved antiviral activity in culture filtrates by mechanical inoculation with tobacco mosaic virus(TMV).HNS2-2 was primarily identified as Streptomyces herbaricolor by morphology under scanning electronic microscope,culture characteristics,chemotaxonomic tests and 16S rRNA analysis.The culture filtrate of HNS2-2 exhibited desirable inhibition effect against TMV.92.62% of lesion number reduction was found on Datura stramonium,a necrosis host of TMV,when TMV was mixed with the 5 time dilution of HNS2-2 culture filtrate for 1 h.83.78% and 67.26% reductions for spraying 5 time dilution of HNS2-2 culture filtrate before or after TMV inoculation respectively.On the other hand,61.88%,54.06% and 42.37% symptom inhibition on the systemic infection host Nicotiana tabacum cv.K326 was observed respectively.更多还原