【摘要】 目的:研究竹康宁中竹叶总黄酮的提取及测定方法。方法:在单因素试验的基础上,采用L9(34)正交设计对乙醇回流提取法和硝酸铝比色测定法进行试验。结果:确定了乙醇回流提取法的最佳提取工艺是:70%乙醇,料液比1∶30,90℃回流提取2次,每次2h。比色法测定3批次竹康宁中的竹叶黄酮,回收率为88.0%92.0%,RSD为1.3%2.2%,样品含量在5.1%5.3%之间。结论:本方法简单,易于推行,结果令人满意。更多还原

【Abstract】 Objective:To study on the methods of extract and determine of bamboo flavone from bamboo leaf essence.Methods:On the basic of monofactorial tests,the methods of extract and determine of bamboo flavone from bamboo leaf essence were studied by the L9(34)orthogonal examination.Results:The best ethanol circumfluence extraction conditions were as follows:leaves:extraction liquid(70% ethanol)=1 ∶30;circumfluence extraction for twice at 90℃ and 2 hours each time.Three batchs bamboo leaf essence were determined by colorimetry.The recoveries were between 88.0% and 92.0% and the RSD were between 1.3% and 2.2%.The content of bamboo flavonoids in them were between 5.1% and 5.3%.Conclusuons:The results are satisfied and the methods are worthy to spread with its easy operation.更多还原