【摘要】 目的研究细胞间黏附分子-1(ICAM-1)在粉防己碱(TET)对抗移植心脏慢性排斥反应中的作用。方法Wistar-Wistar和SD-Wistar大鼠间的颈部心脏移植分别作为同系和异系对照。TET治疗组(SD-Wistar)大鼠每天另给予50mg/kgTET灌胃。异系对照和TET治疗组大鼠每日给予环孢素A灌胃(1.5mg/kg),同系对照组不用任何药物治疗。12周后取移植心脏做组织学检查,免疫组织化学法检测组织中ICAM-1表达,以图像分析仪计算阳性率。结果异系对照动脉管壁占血管直径的平均比值为0.64±0.20,明显高于同系对照(0.23±0.07,P<0.01)和TET治疗组(0.33±0.11,P<0.05);ICAM-1在同系对照低度表达,异系对照的表达明显增强,TET治疗组的ICAM-1表达则明显减少。结论ICAM-1在慢性排斥的发生中具有重要作用,抑制ICAM-1表达可能是TET抗慢性排斥的作用机制之一。更多还原

【Abstract】 Objective To The effect of intercellular adhesion molecule-1(ICAM-1)in chronic rejection investigated treated with tetrandrine.Methods Cervical heterotopic heart transplantation was performed from Wistar to Wistar rats(isograft controls),or SD to Wistar rats(allograft).TET-treated group rats were administered with TET at a dose of 50 mg/kg body weight for 12 weeks continuously.Allograft control and TET-treated groups were also treated with cyclosporine at a dose of 1.5 mg/kg body weight.Isograft control group rats were not treated with any drug.Histological changes were monitored after 12 weeks of operation,expression of ICAM-1 was measured by immunohistochemistry,and the percentages of positive cells were calculated with computer imaging analysis system.Results In allografts,the mean ratio of depth of vessel wall and vessel diameter was 0.64±0.20,much higher than that in isografts(0.23±0.07,P<0.01)and TET-treated group(0.33±0.11,P<0.05).Low-grade ICAM-1 expressed in isograft group,enhanced expression was seen in allograft group,and reduced expression in TET-treated group.Conclusion ICAM-1 plays important role in chronic rejection,and inhibiting ICAM-1 expression of might be the major mechanisms of TET attenuating the development of chronic rejection.更多还原