【摘要】 目的建立间接竞争酶联免疫吸附法测定食品中的黄原胶。方法应用黄原胶抗原免疫的绵羊多克隆抗体,采用间接竞争酶联免疫吸附方法,对黄原胶进行检测分析。结果黄原胶的检测限为0.1ng/ml,有效检测区间为0.1～1×103ng/ml。组间及组内差异均小于10%。烧烤酱和凯撒沙拉酱中的黄原胶检测限为50ng/ml,有效检测区间为50～5×103ng/ml。烧烤酱和凯撒沙拉酱中黄原胶回收率分别为72.0%～89.8%和102.6%～119.0%。结论本方法简单、快速、成本低,重现性好,适合检测食品中的黄原胶。更多还原

【Abstract】 Objective To determine xanthan by enzyme-linked immunosorbent assay(ELISA) in food stuffs.Method Sheep against xanthan polyclonal antibodies have been used in IC-ELISA to detect xanthan.Results The limit of detection for this assay was 0.1 ng/ml for pure xanthan.The active range was between 0.1 and 1×103 ng/ml.Intra-assay and inter-assay coefficients of variation(CV) were lower than 10% within the active range of the assay.The limit of detection for BBQ sauce and Caesar dressing was 50 ng/ml while the active range was between 50 and 5×103 ng/ml.Recovery rates of this assay were between 72.0%～89.8% in BBQ sauce and 102.6%～119.0% in Caesar dressing.Conclusion An IC-ELISA assay was developed for detection of xanthan,which could be simple,rapid,money saving and reliable for detecting xanthan in food stuffs.更多还原