【摘要】 对传代296次的牙鲆(Paralichthys olivaceus)鳃细胞系(FG)进行染色体制备和组型分析,并与牙鲆活体鳃细胞组型进行比较。牙鲆活体鳃细胞染色体数目为48,且均为端部着丝粒染色体,染色体组型公式为2n=48,48t,NF=48。而鳃细胞系的染色体数目出现较大变化,有两个分布区间,一个区间是53~79;另一个是119~139。其模式染色体数为68,在模式细胞分裂相中,有30对端部着丝粒染色体和4对中部着丝粒染色体;染色体条数在119以上的分裂相中,除了有很多中部着丝粒染色体以外,小染色体也以很高的频率出现。通过上述研究分析表明,牙鲆鳃细胞系经多次传代后,其染色体与活体鳃细胞染色体相比存在着明显的差别,已经发生了变异,不适合用于牙鲆染色体核型、组型等方面的研究。更多还原

【Abstract】 The karyotypes of flounder Paralichthys olivaceus gill and flounder gill cell line (FG) which had been subcultured for 296 generations were analyzed and compared with that of live gill cell of P. olivaceus. The chromosomes of flounder gill were all acrocentric chromosomes and the chromosome number was 48; the karyotype formula was 2n=48,48t,NF=48. The chromosome number of FG cell line fluctuated largely. Two interval distribution were identified:one was from 53 to 79,and the other was from 119 to 139. Its modal chromosome number was 68,and the metaphase chromosomes of modal cells included 4 pairs of metacentric chromosomes and 30 pairs of acrocentric chromosomes. Among the metaphases whose chromosome numbers were more than 119 many metacentric chromosomes were detected,and microchromosomes were also found at a high frequency. The results showed that after subcultured for many generations the flounder gill cell line chromosomes had changed apparently compared to flounder gill;the cell line had undergone cell transformation and karyotype evolution,and the chromosomes of FG cell line could not be used for chromosome research of flounder P. olivaceus.更多还原