【摘要】 目的评价不同串联重复序列(VNTR)位点组合在北京地区北京基因型结核分枝杆菌基因分型中的应用。方法采用多位点数目可变串联重复序列分析(MLVA)技术对72株北京地区北京基因型菌株的24个VNTR位点进行分析,基因多态性分析采用BioNumerics软件。结果24个位点的多态性差异较大,位点QUB-11b(HGI 0.651)、Mtub 21(HGI0.556)和QUB-26(HGI 0.518)的多态性较高,有两个位点(MIRU 2和MIRU 24)则不具有多态性;不同VNTR位点组合(12位点,15位点,24位点)在北京基因型菌株中的分辨指数依次升高,分别为0.788,0.990,0.992,后两个组合的差异仅是由位点Mtub 29引起的。结论不同的VNTR位点在北京地区北京基因型菌株中的分辨力存在差异;推荐的基础VNTR 15位点与Mtub 29组合可作为北京地区结核分枝杆菌分子流行病学研究的一线方法。更多还原

【Abstract】 The newly proposed variable-number tandem-repeat(VNTR) typing system,including basic 15-locus set and high-resolution 24-locus set,were evaluated in 72 Beijing genotype Mycobacterium tuberculosis isolated from Beijing area in China.As a result,24 loci showed different discriminatory.in which QUB-11b(HGI 0.651),Mtub 21(HGI 0.556) and QUB-26(HGI 0.518) were the relatively more polymorphic loci,MIRU 2 and MIRU 24 had extremely low resolution among Beijing genotype strains(HGI 0).The most widely used 12-locus set had limited power(HGI 0.788) in this sample collection.A dramatically increased resolution was observed by using the proposed VNTR 15-locus and 24-locus set,with HGI of 0.990 and 0.992,respectively.The subtle difference in HGI was originated from one locus Mtub 29,which was included in 24-locus system.Thus,the proposed VNTR 15-locus plus Mtub 29 can be served as the first screening method in routine epidemiological study in Beijing area.更多还原