【摘要】 目的:观察替米沙坦与福辛普利对心肌梗死后大鼠心脏血管紧张素转换酶2(Angiotensin-convertionenzyme2,ACE2)的影响。方法:结扎大鼠冠状动脉左前降支制作急性心肌梗死模型;将45只SD大鼠随机分为假手术组,心肌梗死组,替米沙坦组,福辛普利组,替米沙坦与福辛普利联用组;手术前后各用药2周;免疫组化检测梗死后2周ACE2蛋白表达;RT-PCR检测梗死后2周ACE2mRNA表达。结果:心肌梗死后2周ACE2蛋白表达显著增加,替米沙坦,福辛普利组ACE2蛋白的表达进一步增加,联合用药组蛋白表达水平低于单药组)。心肌梗死后2周ACE2mRNA未见明显增加,替米沙坦组ACE2mRNA表达显著增加,福辛普利组和联用药组ACE2mRNA表达未见明显增加。结论:替米沙坦,福辛普利均能增加心肌梗死后大鼠心脏ACE2的表达,联合用药不比单用更能增加ACE2的表达。更多还原

【Abstract】 Objective:To investigate the effect of telmisartan and fosinopril on the expression of angiotensin-convertion enzyms 2 (ACE2) in cardiomyocyte after myocardial infarction.Methods:Animal model of acute myocardial infarction were established by ligating the left anterior descending coronary artery of rats. Vehicle, telmisartan,fosinopril,or both drugs combined were giv- en to rats by intubation feeding,from 2 weeks before coronary artery ligation to 2 weeks there after. ACE2 protein expression of myocardium were semi-quantified by SP immunohistochemistry. ACE2mRNA were measured by Reverse Transcriptase Poly- merase Chain Reaction (RT-RCR). Results:Compare with sham operated group,ACE2 protein increased significantly in infarc- tion group,but not the ACE2 mRNA. telmisartan and fosinopril therapy caused a significant increase in ACE2 protein compare to infarction group,but only telmisartan caused a significant increase in ACE2 mRNA though all therapied groups showed higher ACE2 mRNA expression. Conclusion:Both telmisartan and fosinopril induced increases in cardiac ACE2 exprssion, whereas the combination of these two drugs do not associated with higher ACE2 expression.更多还原