【摘要】 目的:探讨水通道蛋白1(Aquaporin1,AQP1)在人羊膜中的表达、定位及意义。方法:应用免疫组织化学方法检测12例正常足月妊娠产妇、11例羊水过少及9例羊水过多者足月妊娠产妇的羊膜组织中AQP1蛋白的表达。结果:AQP1在羊膜组织立方上皮细胞中有阳性表达,且主要位于细胞浆中,而在成纤维细胞中仅有微弱表达。羊水过少及过多患者与正常产妇的羊膜组织AQP1蛋白面积积分光密度值比较差异均有显著性(P<0.05)。结论:人羊膜组织表达AQP1蛋白,其在羊水的液体转运中可能起着重要作用,为羊水的水转运机制研究提供了分子生物学基础,并且为羊水量异常的治疗提供了新的思路。更多还原

【Abstract】 Objective:To investigate the expression,location and significance of aquaporin1(AQP1) in the tissue of amniotic membrane.Methods:Immunohistochemical method was used to examine the expression of aquaporin1 in the tissue of amniotic membrane of 12 normal full-term pregnant women,11 full-term pregnamt woman with oligohydramnios and 9 with polyhydramnios.Result:Aquaporin1 was expressed in cuboidal epithelial cells of amniotic membrane,and mainly in cytoplasm.There was but a little expression of aquaporin1 in fibroblast of amniotic membrane tissue.There was statistically significant difference of the AQP1 expression between oligohydramnios,polyhydramnios and normal full-term pregnancy women integrative optical density in amniotic membrane tissue,respectively(P<0.05).Conclusion:AQP1 is expressed in cytoplasm of amniotic membrane tissue of human,and it may play a critical role in the process of water-transference.This study provides a molecular biological basis for the study the on molecular mechanism of amniotic fluid transport,and a new thinking for treating abnormal capacity for amniotic fluid.更多还原