【摘要】 目的:研究乙肝病毒(HBV)感染者HBV基因型分布状况,并建立一套HBV病毒株分型的简便可靠的分析法。方法:设计型特异引物,采用基因型特异引物聚合酶链反应(PCR)法分型。结果:238株HBV中218株得到分型。其中B型149例(男115例、女34例);C型63例(男47例、女16例);B+C混合型6例(男4例、女2例)。B型、C型和B+C混合型检出率分别为68.3%(男69.3%、女65.3%)、28.9%(男28.3%、女30.8%)和2.8%(男2.4%、女3.8%)。未检出A、D、E、F、G、H基因型。结论:HBV感染者以B基因型为主。基因型B检出率显著高于其它基因型,其差异具有统计学意义(P<0.01)。HBV各基因型分布与性别无关(P>0.01)。更多还原

【Abstract】 Objective:To establish a set of suitable and reliable methods and to study the distribution of HBV genotypes.Methods:Type-specific primers were designed,and type-specific primers PCR was used to genotype the HBV strains.Results:A total of 218 out of the 238 strains were genotyped.Among them,49(68.3%) cases were genotype B(male 69.3%,female 65.3%),63(28.9%) cases were genotype C(male 28.3%,female 30.8%),6(2.8%) cases were mixtures of genotype B and C(male 2.4%,female 3.8%).No genotype A,D,E,F,G and H were found.Conclusion:Genotype B is the most dominant type for HBV strains and there is no relationship between the gender and HBV genotypes.更多还原