【摘要】 利用电喷雾串联质谱(ESI-MSn)技术研究马钱苷的质谱碎裂机理,探讨其碎裂方式,并利用傅立叶变换离子回旋共振质谱(FTICR-MS)及SORI-CID高分辨率、高质量准确度对其碎裂机理进行验证研究。采用H/D交换和串联质谱的方法,发现马钱苷羟基上的活泼氢在质谱中性碎片丢失过程中较易被转移,表现出较大的化学活性。通过加入特定的Na+和Li+等碱金属离子,极大地提高了马钱苷化合物质谱分析的灵敏度,获得了更多的结构信息,建立了马钱苷化合物质谱鉴定和分析的新方法。更多还原

【Abstract】 Loganin was investigated systematically by electrospray ionization tandem mass spectrometry(ESI-MSn) to propose the logical fragmentation pathways.Product ion mass spectra examined at high mass resolution allowed unambiguous determination of the elemental composition of fragment ions by sustained off-resonance irradiation/collision-induced dissociation(SORI-CID) method in fourier transform ion cyclotron resonance mass spectrometry(FTICR-MS).Through hydrogen and deuterium exchange,the fragment path of Loganin is further confirmed.Experiment demonstrates that + and + ions can provide complementary useful structural information for structure analysis of Loganin.更多还原