【摘要】 目的探讨深静脉血栓对非血栓段静脉内皮细胞的损伤作用。方法90只SD大鼠随机分为血栓组、手术对照组和正常对照组。血栓组在左髂静脉以结扎法建立血栓模型;手术对照组开腹显露髂动静脉模拟同等程度创伤,但不做静脉分离和结扎;正常组直接取材。取材标本是未形成血栓的上腔静脉和髂静脉及其酶解下的内皮细胞,送电镜、流式细胞仪凋亡检查。结果血栓组非血栓段内皮细胞形态与手术对照组均有明显改变;血栓组与手术对照比较,1,2,4,7d术后早期凋亡率差异均有统计学意义(均P<0.05,P<0.01)。手术对照组早、晚期调亡率与正常组比较,也有显著差异,但较血栓组程度低,持续时间较短;手术对照组建模后4,7d与正常组比较,晚期凋亡率无统计学意义。结论局部深静脉血栓可以导致其他远隔部位静脉内皮细胞损伤,易发生早期凋亡。更多还原

【Abstract】 Objective To search the effect of venous thrombosis(VT) on the endothelial cells(ECs) of non-thrombosis vein segment(ECNT).Methods Ninty rats were divided into a normal group,a thrombosis group established by ligating a section of iliac vein,and a sham operation control group.An electroscope was used to detect the ECs of target vessels morphological changes.The Ecs were collected from non-thrombotic superior vena cava and iliac veins by enzymolysis and studied by flow cytometer to detect the positive rate of apoptosis.Results The morphology of endothelial cells in the non-thrombus vein segments of the thrombosis group showed marked changes compared to those of sham operation control group.Comparison of thrombosis group and sham aperation control group showed significant difference in early apoptosis rate at 1,2,4,and 7 d after operation(all P<0.05,0.01,P<0.01).Conclusions Local deep vein thrombosis can lead to endothelial cell injury and early apoptosis of distant vein segment.更多还原