【摘要】 目的研究β-七叶皂苷钠(β-aescin,β-A)对大鼠缺血再灌注(I/R)后肠黏膜的保护作用,并探讨其作用机制。方法88只健康雄性Wistar大鼠,随机分为2组:I/R对照组和I/Rβ-A预处理组,每组按I/R不同时点(0,1,2h),观察组织病理损害,Haglund评分,肠含水率,肠毛细血管通透性,肠黏膜上皮细胞调亡率,血清TNF-α,IL-6含量变化及生存率。结果与对照组比较,预处理组的肠黏膜组织病理损害,肠含水率,毛细血管通透性,血清TNF-α,IL-6水平(P<0.01)及肠黏膜细胞凋亡率均明显降低(P<0.05),而且生存率明显提高(P<0.05)。结论β-A对肠I/R后肠黏膜具有保护作用;其机制可能与抑制炎症细胞因子,降低I/R后毛细血管通透性及降低肠黏膜细胞调亡率有关。更多还原

【Abstract】 Objective To investigate the protection effect of β-aescin on rat intestine ischemia /reperfusion(I/R) injury.Methods Eighty-eight male wistar rats were randomly divided into two groups,then they were distinguished by different time point after occurrence of reperfusion at 0 h,1 h,and 2 h.We observed the changes of the serum tumor necrosis factor-α,interleukine-6,the apoptosis of intestine mucosal epithelial cell,intestinal microvascular permeability,and the survival rate.Results The group with β-aescin pretreatment was observed to have reduction of intestinal histopathological damage,and significant decrease of Haglund scores,intestinal microvascular permeability,intestinal water content and the level of TNF-α and IL-6(P<0.01),and inhibition of intestinal mucosa cell apoptosis(P<0.05) and mortality rate during 36 hours(P<0.01) compared to controlled group with I/R after reperfusion.Conclusions The β-aescin pretreatment could protect the intestinal mucosa from I/R injury,and it might be related to suppression of proinflammatory factors,reduction of intestinal mucosa cell apoptosis and decreased intestinal microvascular permeability.更多还原