【摘要】 目的观察缺血后处理对大鼠肝脏缺血再灌注损伤的影响。方法120只Wistar大鼠随机分为缺血再灌注组(IRI),缺血后处理组(IPO)和假手术组(S),于复灌后0,0.5,1,2,4,8,12,24h取材,应用RT-PCR法检测各组c-fos,c-jun mRNA的表达。结果(1)在IRI组再灌注后0.5~2h,c-fos和c-jun的表达均增高,1h达高峰;4h后仅c-jun有持续较高的表达,c-fos的表达开始下降;(2)IPO组各时点c-fos mRNA的表达均较IRI组低,但无统计学差异(P>0.05);(3)与IRI组相比,IPO组c-jun mRNA在0.5,1h和2h组明显降低(P<0.05)。结论缺血后处理能有效地保护肝脏免受缺血再灌注造成的损伤,这种保护效应的机制可能与影响即早基因的转录有关。更多还原

【Abstract】 Objective To investigate the effects of the ischemic postconditioning(IPO) on the ischemia reperfusion injury(IRI) in rat livers.Methods One hundred and tweenty Wistar rats were randomly divided into ischemia reperfusion group(IRI),ischemic postconditioning(IPO) group and sham operation(S) group.Liver tissues were harvested at 0,0.5,1,2,4,8,12 h and 24 h after reperfusion in both IPO and IRI groups.The mRNA expressions of the immediate early gene c-fos and c-jun were detected with RT-PCR methods.Results(1) The expression of c-fos and c-jun both maintained a high level from 0.5 h to 2 h after reperfusion in IRI group,and the peak time occurred at 1 h after reperfusion.After 4 hours of reperfusion,the c-fos expression began to decrease gradually while the c-jun expression still remained high.(2) At every time point,the c-fos expressions in IPO group were lower than IRI group(P>0.05).(3) Compared with IRI group,the c-jun expression at 0.5,1 h and 2 h after reperfusion in IPO group was significantly lower(P<0.05).Conclusions IPO can impact the immediate early gene expression at the very early moment after reperfusion,which may be part of the mechanisms of IPO protection effect on ischemia reperfusion injury.更多还原