【摘要】 目的研究金雀异黄素(genistein,Gen)与5-氟尿嘧啶(5-fluorouracil,5-FU)对人胃癌细胞SGC-7901的抑制作用。方法采用MTT法检测Gen和5-FU对SGC-7901细胞的抑制作用,流式细胞术检测细胞周期分布,并在透射电镜下观察细胞超微结构改变。结果Gen和5-FU单用或联合应用对胃癌SGC-7901细胞的生长均有显著的增殖抑制作用,且呈剂量和时间依赖性。两药联合应用时,当药物效应在0.2~0.8时,具有协同或相加作用(CI≤1);18.8μmol/L的Gen作用后,62.97%的SGC-7901细胞阻滞在G2/M期;8.84μmol/L的5-FU作用后,63.76%的SGC-7901细胞阻滞在S期;3.06μmol/L的Gen与7.96μmol/L的5-FU联合应用后,67.46%的SGC-7901细胞阻滞在G0/G1期。Gen和5-FU均可诱导SGC-7901细胞凋亡。结论Gen与5-FU通过阻滞细胞周期和诱导细胞凋亡而抑制胃癌细胞的生长,对胃癌细胞的增殖抑制具有协同作用。更多还原

【Abstract】 Objective To study the effect of genistein(Gen)in combination with 5-fluorouracil(5-FU)on human gastric carcinoma cells in vitro.Methods Human gastric carcinoma cell line SGC-7901 was treated with Gen and 5-FU alone or in combination for 24,48 and 72 hours,respectively.MTT assay was used to investigate the inhibitory effect of Gen and 5-FU on SGC-7901 cells.Transmission electron microscopy was used to observe the ultramicrostructure changes of cancer cells and the flow cytometry was used to detect the distribution of cell cycle.Results Gen and 5-FU alone or in combination inhibited the proliferation of SGC-7901 cells significantly in both time-dependent and dose-dependent manner.The combination of Gen with 5-FU had synergistic effect on the growth of SGC-7901 cell line when the inhibition ratio was 20% to 80%.When SGC-7901 cells exposed to 18.8 μmol/L Gen,8.84 μmol/L 5-FU,and 3.06 μmol/L Gen combined with 7.96 μmol/L 5-FU for 72 hours,62.97%,63.76% and 67.46% SGC-7901 cells were arrested in G2/M,S and G0/G1 phrase,respectively.Gen and 5-FU could both induce apoptosis and arrest cell cycle of SGC-7901 cells.Conclusion Combination therapy of Gen with 5-FU may take synergistic inhibitory effect on the proliferation of gastric cancer cells by resting cell cycle and inducing cell apoptosis at a certain range of concentration.更多还原