【摘要】 【目的】研究黄瓜花叶病毒病(CMV)侵染胁迫下黄瓜重要功能基因表达和代谢途径响应。【方法】在结合气体交换和叶绿素荧光参数及抗性相关酶活性测定的基础上,应用农业部园艺植物生长发育与品质调控重点开放实验室自主开发的黄瓜cDNA芯片研究黄瓜在CMV侵染胁迫下的基因表达谱变化,并对其中5个具有代表性的基因通过实时荧光定量PCR(QRT—PCR)进行检测,以验证cDNA芯片杂交结果的可靠性。【结果】共获得67个差异表达显著的基因,其中42个基因下调表达,25个基因上调表达。这些差异表达的基因涉及了许多重要代谢途径。许多与光合作用、氮同化相关的基因受到明显的抑制;而一些防御相关基因和信号传导相关基因则诱导表达;同时,也发现了一些与CMV胁迫相关的功能未知基因或未有任何功能信息的基因。【结论】CMV胁迫引发了黄瓜代谢发生一系列复杂的适应性变化,PR1-1a等基因可能在黄瓜防御CMV侵染过程发挥着重要作用。更多还原

【Abstract】 【Objective】 The aim of this paper is to study on the expression of important functional genes and pathways in cucumber leaves in response to CMV stress.【Method】On the basis of study of photosynthesis and activities of defence-related enzymes, the changes of gene expression profiles was analyzed by microarray in cucumber (Cucumis sativus L.) after CMV infection. In order to validate the microarray result, five representative clones induced or repressed from microarray analysis were selected for real-time quantitative PCR analysis. 【Result】 Significant changes in transcript abundance were observed for 67 genes, among them, 42 showed down-regulated expression and 25 showed up-regulated expression. Analysis of these CMV-responsive genes showed that they belong to wide range of functional categories. Many genes for photosynthesis and nitrogen assimilation were strongly repressed while some defense-related genes, on the other hand, were found to be up-regulated. In addition to these expression changes, some functions unknown or unreported novel genes that respond to CMV stress were also identified.【Conclusion】 A complex set of metabolic adaptation appears to occur during CMV stress and some genes, such as PR1-1a might play an important role in defence.更多还原