【摘要】 【目的】将抗虫基因转入栽培大豆中,提高大豆的抗食心虫能力。【方法】利用大豆未成熟子叶体细胞胚发生系统高效转化体系,将携带有人工合成的CryIA杀虫基因和CpTI基因的高效双价杀虫基因植物表达载体pGBI121S4ABC转化到大豆主栽品种吉林20与吉林27中。【结果】GUS活性分析、PCR、Southern blot检测证明,目的基因已整合到受体大豆基因组中。T1代转基因大豆抗虫测定表明,转基因材料虫食率比对照显著降低,抗虫能力显著提高。【结论】T3～T5连续3代接虫测定结果表明,转基因大豆株系0-195和0-150的虫食率均比对照低大约30%,其抗虫能力已基本稳定。更多还原

【Abstract】 【Objective】The objective of this study is to obtain highly insect-resisant transgenic soybean plants containing both CryIa and CpTI genes. 【Method】Bivalent insect resistant genes CryIA and CpTI were transformed into immature cotyledon of soybean varieties JILIN 20 and JILIN 27 which have high frequency of somatic embryogenesis via Agrobacterium-mediated. 【Result】 GUS analysis, PCR and Southern blot analysis showed that the foreign gene was integrated into the chromosomal genome of receptor parent. 【Conclusion】 Analysis of insect resistance of successive three generations of transgenic soybean indicated that their resistance has been basically stable.更多还原