【摘要】 【目的】筛选出大白菜细胞核显性雄性不育基因的连锁标记,利用该标记对显性不育基因跟踪、鉴定,提高选择效率,缩短转育周期,加快育种进程。【方法】以大白菜细胞核显性雄性不育基因的分离群体B00160([938A×太NB]-2X3-1X2)的115个单株为试材,采用BSA方法和RAPD技术,对400个随机引物进行筛选。【结果】获得了一个与核基因显性雄性不育基因连锁距离为1.74cM的RAPD标记M264300。将该多态性片段克隆、测序和序列分析,设计了一对长20bp的特异性引物,成功地将RAPD标记转化成为SCAR标记SM264300。该标记与雄性不育基因的遗传连锁距离为2.61cM。利用该SCAR标记对另外2份育性分离群体的不育基因进行检测,准确率达到100%。【结论】该标记可用于大白菜核显性不育基因转育过程中的辅助选择。更多还原

【Abstract】 【Objective】Dominant genic male sterility has been playing an increasingly inportant role for efficient production of hybrids in Chinese Cabbage [Brassica rapa L.ssp pekinensis (Lour.) Olsson]. Using marker assisted selection of the dominan male sterile gene can accelerate breeding programs. 【Method】 Bulked segregant analysis was used to identify RAPD markers linked to a dominant male sterile gene in the population of ‘B00160’, a sterile and fertile segregating populatuon in Chinese cabbage 【Result】One RAPD marker, M264300 was identified in the segregating population tightly linked the gene. The polymorphic fragments were cloned and sequenced. A sequence characterized amplified region (SCAR) marker was devveloped besed on the nucleotide sequence information. The SCAR marker showed the same size and pattern of segregation as the original RAPD marke with a map distance of 2.61 cM and the RAPD maker was converted into SCAR maker. 【Conclusion】 The marker developed here will facilitate the breeding for new elite homozygous sterile lines of Chinese cabbage.更多还原