【摘要】 【目的】研究经静脉注射途径进入体内的不同形态锰在肉仔鸡体内代谢利用上的差异。【方法】将180只22日龄肉公鸡按体重随机分为5个处理组,分别翅静脉注射生理盐水,或在生理盐水中溶解硫酸锰及弱、中等和强络合强度有机锰源的注射液20d。【结果】静脉注射后第10和20天,各注射锰源组鸡的跖骨灰锰含量、心肌锰含量、心肌细胞线粒体中MnSOD活性和MnSOD mRNA均显著(P<0.03)高于注射生理盐水的对照组,但前3项指标未能恒定一致地反映出不同形态锰对肉仔鸡的生物学活性的差异。注射中等络合强度锰源组心肌细胞线粒体中MnSOD mRNA水平显著高于注射硫酸锰组(P<0.01)和强络合强度锰源组(P<0.09),且注射弱络合强度锰源组心肌细胞线粒体中MnSOD mRNA水平显著高于注射硫酸锰组(P<0.06)。【结论】注射锰在转录水平上显著影响心肌细胞线粒体中MnSOD的基因表达,这一功能性基因表达指标比其酶活性等其它指标更敏感、更恒定地监测出肉仔鸡对不同锰间生物学利用性的差异。经静脉注射途径进入体内的中等和弱络合强度有机锰源对肉仔鸡的生物学活性显著高于无机锰,而强络合强度有机锰源不利于肉仔鸡体组织细胞对其中锰的利用。更多还原

【Abstract】 【Objective】Differences in Mn metabolic utilization between organic and inorganic Mn sources or among organic Mn sources for broilers were detected. 【Method】 Broilers at 22 day-old were randomly allocated into five treatment groups and injected with 0.9% NaCl solution (control), or the 0.9% NaCl solution dissolved with Mn sulfate, or organic Mn source with weak, moderate, or strong chelation stength for 20 days. 【Result】On d 10 and 20 after injection, chicks injected with Mn containing solution had a significantly higher metatarsal bone ash Mn content, heart Mn content, heart MnSOD activity, and MnSOD mRNA level than those in control group (P<0.03), but the indices except MnSOD mRNA level could not consistently detect the differences of bioavailabiliy among Mn sources. Chicks injected with solution containing organic Mn source with moderate chelation strength had higher heart MnSOD mRNA level than those injected with solution containing Mn sulfate (P<0.01) or organic Mn source with strong chelation strength (P<0.09), and chicks injected with solution containing organic Mn source with weak chelation strength had higher heart MnSOD mRNA level than those injected with Mn sulfate (P<0.06). 【Conclusion】The results indicated that intravenously injected Mn could significantly affect heart MnSOD gene expression transcriptionally, and this functional index could more sensitively and consistently detect the differences of bioavailability among Mn sources than other indices. Intravenously injected Mn from the organic Mn source with moderate or weak chelation strength was utilized and functioned in the sensitive target tissue of broilers more effectively than Mn from Mn sulfate, and the organic Mn source with the strong chelation strength would be not good for tissue utilization.更多还原