【摘要】 克隆并分析猪FcRn基因;十二指肠组织提取总RNA,利用设计的引物进行RT-PCR,PCR产物与pMD-19T载体连接后转化JM109感受态细胞,筛选阳性克隆并测序;FcRn阳性克隆两条序列在NCBI上比较,显示序列同猪FcRn(AY740682.1)同源性都为99%。克隆了猪FcRn基因的序列和其剪接变体序列,其剪接变体序列已在GenBank上注册(Accession.EU852582)更多还原

【Abstract】 To clone and analysis the FcRn gene of pig.Total RNA was extracted from mucosal tissue of duodenum and mRNA sequence of gene were amplified by RT-PCR using two designed primers.The PCR products were ligated into the pMD-19T vector, and then transformed into competent cells of JM109.The sequence was analyzed to identify the recombinant plasmid.Identity analysis showed that the two FcRn nucleotide sequence shared 99 % .The porcine alternatively spliced variant of FcRn gene was successfully cloned in the present study and the sequence of spliced variant has been submitted to GenBank(Accession.EU852582).更多还原