【摘要】 从辣椒cDNA文库中克隆获得一个推定的WRKY(CaWRKY)转录因子,为分析其功能,分别构建启动子区含有双倍W(2W)盒的报告载体和WRKY置于2×35S启动子控制下的超表达载体,通过基因枪瞬间转化技术将两种载体共转化洋葱表皮,检测共转化细胞中GUS基因的表达情况来分析转录因子与特定顺式作用元件的结合及对其转录的激活效应。结果表明CaWRKY能与W盒结合,表明该基因是WRKY转录因子的编码基因。更多还原

【Abstract】 The specific binding activity of a putative WRKY transcription factor to W-box was analyzed by transient expression with a reporter vector harbored double W box and an effect vector with the putative WRKY transcriptional factor driven by double CaMV35s promoter.The reporter vector and effect vector were constructed and then mixed and cotransformed into onion epidermal cell through particle bombardment.GUS blue dots were observed in onion epidermal cells 24h after transformation.The result indicated that the putative WRKY transcriptional factor can bind to cis-element W box,it maybe WRKY transcriptional factor of Capsicum annuum.更多还原