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细菌纤维素耐低温生产菌株的诱变选育
Mutation and selection of low temperature-resistant bacteria for cellulose production
【摘要】 以实验室保存的木醋杆菌(Acetobacterxylinum)为出发菌株,利用紫外诱变的方法,以获取在低温条件下能高产细菌纤维素的突变株。通过初筛和复筛以及连续传代实验,确定了1株突变性状能稳定遗传的突变株,产量可达8.6g/L(干重),比原出发菌株产量提高了20%。
【Abstract】 The Acetobacter xylinum strain preserved by our laboratory,as the original strain,was mutated using UV mutagenesis in order to obtain a low temperature-resistant and high cellulose-producing mutant strain.One mutant strain,which was steady in hereditary,was obtained by preliminary screening and second screening,as well as serial passage.The yield of cellulose by the mutated strain reached 8.6 g/L in dry weight,which was 20% higher than that produced by the starting strain.
【关键词】 紫外诱变;
细菌纤维素;
低温;
木醋杆菌;
【Key words】 UV mutagenesis; bacterial cellulose; low temperature; Acetobacter xylinum;
【Key words】 UV mutagenesis; bacterial cellulose; low temperature; Acetobacter xylinum;
【基金】 山东省自然科学基金(Q2006F08);上海市博士后基金(06R214106)
- 【文献出处】 中国酿造 ,China Brewing , 编辑部邮箱 ,2008年17期
- 【分类号】Q933
- 【被引频次】6
- 【下载频次】206