【摘要】 以罗非鱼皮为原料,采用直接酶法提取胶原蛋白肽,用正交试验对罗非鱼皮胶原蛋白肽的制备工艺进行优化,并测定了罗非鱼皮的基本组成成分和鱼皮胶原蛋白肽的氨基酸组成。结果表明,碱性蛋白酶添加量为0.5%,固液比1:3,酶解时间为3h,酶解温度50℃时鱼皮胶原蛋白肽的提取率最高为13.5%。并对产品胶原蛋白肽的抗氧化特性进行了分析。更多还原

【Abstract】 The collagen peptides of tilapia skin has been extracted by using alcalase method.The technological parameters of the extraction were optimized by orthogonal test.The composition of tilapia skin and the amino acid composition of the collagen peptides were also analyzed.The results showed that the optimal addition amount of alcalase was 0.5%,the enzymatic hydrolysis time was 3 h,hydrolysis temperature was 50℃ and the ratio of fish skin to solvent was 1:3.Under above conditions,the extraction yield of collagen peptides from tilapia skin was 13.50%.The antioxygenic property of collagen peptides was also analyzed.更多还原