【摘要】 以球形红假单胞菌1.1737T为出发菌株,经紫外线和亚硝基胍诱变,并结合罗红霉素抗性和耐前体性物质-对羟基苯甲酸抗性突变株的筛选,获得1株辅酶Q10高产菌株PHBr-2,该菌株经发酵108 h的生物量为15.7g/L,辅酶Q10产量达到50.6mg/L,比原始菌株在同样条件下产量提高了90.9%。该变异株遗传性能稳定,经转接5代后其辅酶Q10产量为49.5mg/L,是1株很有前景的辅酶Q10生产菌株。更多还原

【Abstract】 Rhodopseudomonas sphaeroides 1.1737T was used as an original strain in this study R.sphaeroides 1.1737T was mutagenized by UV andnitrosoguanidine(NTG) treatment.Then,a high coenzyme Q10-producting mutant R.sphaeroides PHBr-2 was screened according to the tolerance onroxithromycin and precursor p-hydroxyben-zoic acid(PHB).Using R.sphaeroides PHBr-2 for fermentation,the biomass reached 15.7 g/L at 108 h,and the coenzyme Q10 yield was 50.6 mg/L(90.9% higher than that of the parent strain).R.sphaeroides PHBr-2 was genetic stable and produced49.5 mg/L coenzyme Q10 after five-generation subculture.It was indicated that R.sphaeroides PHBr-2 was a good candidate for coenzyme Q10production.更多还原