【摘要】 目的:了解猪苓多糖(PPS)及猪苓多糖联合卡介苗(BCG)体内刺激对小鼠腹腔巨噬细胞TLRs表达的影响。方法:应用流式细胞术检测PPS联合BCG体内刺激2、12、24小时后小鼠腹腔巨噬细胞表面CD14、TLR2、TLR4及粘附分子CD11b的表达。结果:PPS组在作用的3个时点内小鼠腹腔巨噬细胞TLR分子表达无明显变化,联合BCG作用2小时后TLR表达高于BCG和PPS单用组;PPS组作用12小时后,CD11b表达高于对照组,24小时后下降,联合BCG作用2小时和12小时,CD11b表达高于BCG和PPS单用组。结论:PPS体内单独刺激不能促进TLR分子的表达,联合BCG可促进TLR分子和粘附分子CD11b的表达。更多还原

【Abstract】 Objective:To learn the effect of injection with PPS and/or BCG on the expression of TLRs in mouse peritioneal macrophages.Methods:Flow cytometry was used to measure the expression of CD14,TLR2,TLR4 and CD11b on mouse peritoneal macrophages 2,12,24 h respectively after injection with PPS and/or BCG.Results:In PPS group,expression of TLRs on mouse peritoneal macrophage did not make significant difference among 3 timepoints,while combined with BCG,PPS induced higher expression of TLRs at 2 h timepoint,compared to injection with BCG or PPS alone.Injection with PPS induced higher expression of CD11b than that with salt at 12 h timepoint,then the expression went down at 24 h timepoint.Combined with BCG induced higher expression of CD11b than that with BCG or PPS alone at 2 h and 12 h timepoints.Conclusion:Injection with PPS alone dose not enhance the expression of TLRs on mouse peritoneal macrophages,while combined with BCG,PPS would induce higher expression of TLRs and CD11b.更多还原