【摘要】 目的建立化学发光酶免疫分析法测定血清前列腺特异性抗原,用于临床前列腺癌、前列腺增生、前列腺炎患者病情的监测和预后.方法化学发光酶免疫分析法(CLEIA)采用辣根过氧化酶(HRP)-鲁米诺竞争法.结果该方法的灵敏度为0.01μg/L,在0.5~80μg/L的范围内线性良好.CLEIA测定血清前列腺特异性抗原的批内、批间与日间变异系数分别为5.0%,4.97%和4.93%.在血红蛋白浓度<2.4 g/L、总胆红素浓度<342μmol/L、甘油三酯浓度<9.9 mmol/L时的干扰率无临床意义.与放射免疫分析法有较好的相关性(r=0.947 2).结论CLEIA法测定血清前列腺特异性抗原能及时有效地满足前列腺癌、前列腺增生、前列腺炎患者的临床需要,有助于进一步对检测试剂盒的研制.更多还原

【Abstract】 Objective To establish the CLEIA for determination of serum prostate specific antigen,prostatic cancer,prostatic hyperplasia and monitoring the condition and prognosis.Methods CLEIA HRP—Isoluminol competitive method.Results The sensitivity was(0.01 μg/L),the assay exhibited a good linear correlation within the range of(0.5)～(80 μg/L),within-run and between-day coefficients of variation were(5.0%),(4.97%) and(4.93%) respectively.The interference of hemolysis Hb (<2.4 g/L),TBil(<342 μmol/L),TG(<9.9 mmol/L) had no clinical significance.The correlation degree between the assay and RIA was highly significant(r=(0.947 2)).Conclusion CLEIA for determination of serum prostate specific antigen can satisfy the clinical requirements of prostatic canner,prostatic hyperplasia effectively,which will be helpful to develop an assay kit.更多还原