【摘要】 目的探讨缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)、p53、CD31在黑色素移植瘤血管生成拟态(vasculogenesis mimic-ry,VM)形成中的作用。方法制作小鼠黑色素移植瘤动物模型,成瘤后每天随机抽取1只小鼠处死,共12 d,获得肿瘤样本114例;HE染色、HIF-1α、VEGF、p53、CD31免疫组化染色和PAS染色。动态观察HIF-1α、VEGF、p53、CD31在黑色素移植瘤VM中的表达。结果在移植瘤形成的1~8 d,肿瘤组织内存在VM;9~12 d移植瘤中的VM被内皮依赖性血管替代;VM周围肿瘤细胞中CD31、HIF-1α阳性百分率高于远离VM区域的阳性百分率(P<0.01);内皮依赖性血管周围肿瘤细胞CD31阳性百分率高于远离内皮依赖性血管区域的阳性百分率(P<0.01);VM周围肿瘤细胞VEGF、p53阳性百分率高于内皮依赖性血管周围的阳性百分率;VM密度与VM周围肿瘤细胞CD31、HIF-1α、VEGF阳性百分率之间存在相关;内皮依赖性血管密度只与内皮依赖性血管周围肿瘤细胞中CD31阳性百分率之间存在相关。结论移植瘤中VM是暂时性血液供应方式,渐被内皮依赖性血管替代;HIF-1α、VEGF、p53、CD31均与黑色素移植瘤VM形成有关,其中CD31、HIF-1α作用尤其重要。更多还原

【Abstract】 Objective To study the effect of HIF-1α,VEGF,p53 and CD31 on vasculogenic mimicry in the melanoma transplant tumour.Methods The mouse melanoma transplant tumor model was made with B16 melanoma cell suspending liquid.When tumor appears,one mouse was chosen each day randomly to execute.114 cases tumor specimens were obtained for 12 days totally to conduct HE stain,immunohistochemistry stain for HIF-1α,VEGF,p53 and CD31 and CD31-PAS double stain.Results The vasculogenic mimicry and endothelial-depended vessel co-exist in the tumor tissue at the early phase of melanoma;From day 9 to day 12,this structure was replaced by endothelial-depended vessel.The number of CD31,HIF-1α positive cell around vasculogenic mimicry were more than that of far away vasculogenic mimicry.The ratio of CD31 positive cell around endothelial-depended vessel had significant difference with that of far away from it.The number of VEGF,p53 positive cell around vasculogenic mimicry were more than that of around endothelialdepended vessel.There were correlation between vasculogenic mimicry density and the number of CD31,HIF-1α,VEGF positive cells around vasculogenic mimicry. The density of endothelial vessel had correlation with the number of CD31 positive cell around endothelial-depended vessel.Conclusions The vasculogenic mimicry is one sort of temporary blood-providing pattern.The HIF-1α,VEGF,p53,CD31 all take part in the development of vasculogenic mimicry and CD31,HIF-1α play key role in this procession.更多还原