【摘要】 目的探讨抑癌基因p53第4外显子第72位密码子(codon72)与人类长寿的关系。方法应用PCR-RFLP技术对巴马156例(年龄90～105岁,平均93.2岁,长寿组)健康壮族长寿老人和142例健康壮族成年人(年龄20～72岁,平均34.8岁,对照组)的p53基因进行分型,了解这些基因的多态性,分析该基因(型)频率在长寿组和对照组之间的差异及其与长寿的关系。结果长寿组p53codon72片段的野生型(G/G)频率明显高于对照组(53%:29%,P<0.05)。结论p53基因的多态性与人类长寿有着较为密切的联系,其具体作用机制有待进一步研究。更多还原

【Abstract】 Objective To explore the association between the polymorphism of tumor suppression gene p53 coden 72 and human longevity.Methods P53 codon 72 were genotyped with PCR-RFLP technique for 156 Zhuang healthily long-living people(aged 90～105,mean 93.2 years,longevous group) and 142 healthy controls(20～72,mean 34.8 years,control group) from Bama area to analyze the polymorphic genotype frequencies.Results Analyses of p53 codon 72 genotype showed that,any simultaneous deletion or mutation of the gene was found more frequent in control group than that in longevous group(53%:29%,P<0.05).Conclusions The tumor suppression gene p53 codon 72 might involve in the human longevity in Bama area which mechanism needs further studies.更多还原