【摘要】 目的研究血小板内皮细胞黏附分子-1(PECAM-1)基因第3外显子C+373G单核苷酸多态性及血浆可溶性PECAM-1(sPECAM-1)水平与严重冠状动脉粥样硬化的相关性。方法入选经冠状动脉造影诊断为三支病变的冠心病患者97例和冠脉狭窄<50%的同期非冠心病患者89例。采用Pyrosequencing基因测序法检测PECAM-1第三外显子C+373G单核苷酸多态性;采用酶联免疫吸附试验方法测定血浆中的sPECAM-1浓度。结果(1)冠心病组(CAD组)中GG型的比例显著高于对照组(26.3%:14.6%,P=0.047)。(2)CAD组sPECAM-1显著低于对照组〔(61.14±34.57)μg/L vs(33.62±38.58)μg/L,P<0.001)。(3)在所有研究对象中,血浆sPECAM-1水平在各基因型中的分布具有一定的趋势〔CC:(41.68±33.60)μg/L;CG:(47.17±38.15)μg/L;GG:(53.18±46.51)μg/L,P=0.433)。冠心病亚组中,sPECAM-1水平在基因型中的分布达到统计学上的差异〔(CC:(23.64±16.90)μg/L;CG:(27.68±30.16)μg/L;GG:(53.97±54.90)μg/L;P=0.012〕。结论PECAM-1C±373G位点的突变是严重冠脉粥样硬化的一个遗传易患因子。sPECAM-1同冠心病具有一定的相关性。更多还原

【Abstract】 Objective To explore the association of platelet endothelial adhesion molecule-1(PECAM-1) C+373G polymorphism and plasma soluble PECAM-1(sPECAM-1) with severe coronary atherosclerosis.Methods The study group consisted of 97 coronary artery disease(CAD) patients with three diseased main coronary arteries,and the control group included 89 patients with coronary artery stenosis<50% diagnosed by coronary angiography during the same time.One single nucleotide polymorphisms(SNPs) of PECAM-1 gene C+373G at exon 3 was analyzed by pyrosequencing method.Level of plasma sPECAM-1 was measured by ELISA.Results The percentage of GG genotype of C+373G polymorphisms in CAD group was significantly higher than that in control group(26.3%:14.6%,P=0.047).The level of sPECAM-1 was found to be decreased in CAD patients compared with control ones((61.14±34.57) vs(33.62±38.58)μg/L,P<0.001)).Moreover,subjects with the homozygous GG genotype of C+373G polymorphisms had higher sPECAM-1 levels,followed by GC and CC genotypes in all subjects(CC:(41.68±33.60)μg/L;GC:(47.17±38.15)μg/L;GG:(53.18±46.51)μg/L;P=0.433).However,in CAD subgroup,the levels of sPECAM-1 in GG genotype are significantly higher than those in GC and CC genotypes(CC:(23.64±16.90)μg/L;CG:(27.68±30.16)μg/L;GG:(53.97±54.90)μg/L;P=0.012).Conclusion The mutation of PECAM-1 C+373G at exon 3 may be a genetic risk factor for severe coronary artery disease in Chinese population.The mean level of sPECAM-1 is associated with CAD.The study suggests that PECAM-1 play an important role in the development of atherosclerosis.更多还原