【摘要】 目的:探讨大鼠全脑缺血再灌注时脑组织血红氧化酶-1(HO-1)活性及全血炭氧血红蛋白(COHb)含量的变化在脑损伤中的作用。方法:42只SD大鼠分为假手术组(S组)7只,脑缺血再灌注组(I组)35只。将I组大鼠制作为全脑缺血再灌注模型,模型成功后1 h,处死S组大鼠;I组分别于1、3、12、24及48 h时各处死7只,分别检测各组大鼠脑组织HO-1活性及一氧化碳(CO)和cGMP含量;计数海马CA1区单位面积神经元存活数。结果:①模型建立1 h时脑组织HO-1、COHb及cGMP含量即达高峰,后逐渐降低,于3 h后又逐渐升高,至48 h再次达第2次高峰。②与S组比较,I组海马CA1区神经元存活数在模型建立1 h时无明显变化,3 h后显著减少,至48 h仍维持于1较低水平。结论:脑缺血再灌注时脑内HO-1高度表达,内源性CO水平升高,提示HO-CO-cGMP系统可能在脑缺血再灌注损伤中起重要作用。更多还原

【Abstract】 Objective: To observe the change of hemeoxygenase-1(HO-1) activity in cerebral tissue,carboxyhemoglobin(COHb) in blood of rats during global cerebral ischemia-reperfusion.Methods: Cerebral ischemia-reperfusion model was produced in SD rats.In sham(S) group and ischemia-reperfusion(I) group HO-1 activity,cyclic GMP(cGMP) in the brain and COHb in blood were evaluated respectively at 1,3,12,24 and 48 h during global cerebral ischemia and reperfusion,and the surviving number of pyramidal cells in hippocampus CA1 was assessed. Results:(1) HO-1 activity,COHb content and cGMP levels in I group peaked at 1 h during global cerebral ischemia and reperfusion,then decreased gradually,and at 3 h began to increase,peaked more at 48 h.(2) As compared with S group,the surviving number of pyramidal cells in hippocampus CA1 of I group did not change significantly at 1 h,then decreased markedly,lasted till 48 h.Conclusion: HO-1 was highly expressed in brain of rats during global cerebral ischemia-reperfusion,and endogenous carbon monoxide increased and HO-CO-cGMP system may play an important role in the pathogenesis of global cerebral ischemia-reperfusion.更多还原