【摘要】 目的探讨RhoC基因过量表达对内皮细胞体外迁移及血管生成过程的影响。方法构建RhoC真核表达载体,以脂质体转染人脐静脉内皮细胞(HUVE),采用RT-PCR和Western blot检测RhoC的mRNA及蛋白表达水平;划痕实验检测细胞迁移能力;胶原基质胶三维培养检测内皮细胞体外血管生成能力。结果转染RhoC实验组与空载体对照组比较,RhoC mRNA和蛋白表达明显增高;细胞迁移能力和血管样结构生成能力明显增强。结论RhoC高表达能促进人脐静脉内皮细胞体外迁移及血管生成。更多还原

【Abstract】 Objective To investigate the effects of RhoC overexpression on human umbilical vein endothelial(HUVE)cell migration and angiogenesis in vitro.Methods Transfecting RhoC gene into HUVE cells by Lipofectamine-2000.The expression of RhoC mRNA and protein were detected respectively by RT-PCR and Western blot,respectively.Wound assay in vitro and three-dimensional culture were used to detect the migration and angiogenesis capacity of HUVE.Results RhoC expression was increased by transfecting RhoC gene into HUVE,meanwhile,the cells migration and angiogenesis capacity were enhanced.Conclusion RhoC may increase the capacity of HUVE migration and angiogenesis in vitro.更多还原