【摘要】 目的探讨PKC-α,CyclinD1和Cdk4在大肠癌中表达的相关性及其与临床病理学特征的关系。方法应用免疫组化SP法对83例大肠组织中PKC-α,CyclinD1和Cdk4的表达进行检测。结果PKC-α在大肠癌组织中阳性表达31/47(66.0%)与正常组织3/15(20.0%)及腺瘤10/21(47.6%)相比差异有统计学意义(P<0.05),与大肠癌分化程度,Dukes分期,淋巴结转移及CEA水平明显相关(P<0.05)。Cy-clinD1和Cdk4在大肠癌中阳性表达分别为26/47(55.5%)、28/47(59.6%),与正常组织中2/15(13.3%)、1/15(6.70%)及腺瘤组织中8/21(38.1%)、9/21(42.9%)相比呈递增趋势(P<0.05)。大肠癌中CyclinD1阳性表达与肿瘤分化程度,Dukes分期,淋巴结转移呈正相关(P<0.05),而与CEA水平关系不大(P>0.05);Cdk4阳性表达则与分化程度,Dukes分期有关(P<0.05),与淋巴结转移及CEA水平关系不密切(P>0.05)。PKC-α与CyclinD1呈正相关关系(r=0.348,P<0.05),CyclinD1和Cdk4呈正相关关系(r=0.393,P<0.05),PKC-α和Cdk4则无明显相关性(r=0.167,P>0.05)。结论蛋白激酶C-α与CyclinD1及Cdk4的过表达在大肠癌的发生、发展中起着重要的协同作用。三者的过表达与大肠癌的分化程度,Dukes分期密切相关。更多还原

【Abstract】 Objective To investigate the relationship between the expression of PKC-α、CyclinD1 and Cdk4 and the clinical significance in colorectal carcinomas.Methods Immunohistochemical staining was used to detect the expressions of PKC-α,CyclinD1 and Cdk4 in normal colorectal tissues,adenoma and carcinoma,respectively.Results The Positive rates of PKC-α expression in normal colorectal tissues,adenoma and carcinomas were 20%,47%,66.0%,respectively(P<0.05),associated with the tumor cellular differentiation,Dukes phase and lymph node metastasis and CEA,respectively(P<0.05).Overexpression of CyclinD1 and Cdk4 were 55.3%,59.6% in colorectal carcinomas,showed an increasing tendency in colorectal carcinogenesis,respectively(P<0.05);the expression of CyclinD1 was correlated closely with differentiated degree,Dukes phage,lymph node metastasis and CEA,respectively(P<0.05);the expression of Cdk4 was associated with differentiated degree,Dukes phase and CEA,respectively(P<0.05).There is a positive relationship between PKC-α and CyclinD1(r=0.348,P<0.05),There is a positive relationship between CyclinD1 and Cdk4(r=0.393,P<0.05).Conclusion Overexpression of PKC-α、CyclinD1 and Cdk4 may contribute to the pathogenesis of colorectal carcinoma,and it was correlated closely with differentiated degree,Dukes phase and CEA.更多还原