【摘要】 利用合成的中等分子量的线性聚丙烯酰胺(LPA)和羟乙基纤维素(HEC)构成的共混聚合物网络对标准DNA样品BigDyeTeminatorV3.1进行测序研究。研究了LPA/HEC的比例,温度,电压等对分离效果的影响。实验证明该聚合物网络同时具有LPA对DNA优良的筛分性能和HEC对毛细管内壁动态涂覆的能力。用2.5%w/v的这种介质在没有对软件系统优化的情况下,73min内对标准DNA的读写长度可达900个碱基。更多还原

【Abstract】 A network formed by linear polyacrylamide (LPA) and hydroxyethylcellulose (HEC) is designed and tested for DNA sequencing on Bigdye Terminator V 3.1 standard DNA sample by capillary electrophoresis (CE). The performance of matrix on DNA sequencing is determined by LPA to HEC molar ratio and sequencing temperature. The results show that under the appropriate ratio of HEC to LPA, the networks yield a read length of up to 900 bases of contiguous sequence in 73 min without optimized base-calling software.更多还原