【摘要】 目的检测缺氧诱导因子-1α(HIF-1α)和环氧合酶-2(COX-2)mRNA及蛋白在大鼠放射性口腔黏膜炎组织中的表达水平,并探讨二者之间的关系。方法建立放射性口腔黏膜炎SD大鼠模型,切取左、右侧颊黏膜,用半定量RT-PCR检测各标本HIF-1α和COX-2的mRNA相对表达值,采用免疫组化SABC法检测HIF-1α和COX-2蛋白在黏膜炎组织中的表达部位及表达强度。结果成功地建立了SD大鼠放射性口腔黏膜炎动物模型;RT-PCR结果显示ROM黏膜组织能够表达HIF-1αmRNA和COX-2mRNA,而右侧自身对照组基本不表达HIF-1α和COX-2mRNA;免疫组化结果显示:HIF-1α蛋白和COX-2蛋白在ROM黏膜组织中均能广泛表达,HIF-1α和COX-2蛋白的表达基本处于平行的关系,并且表达的部位和细胞类型也基本一致。结论放射性口腔黏膜炎组织有HIF-1α和COX-2mRNA及蛋白的表达,两者的表达呈高度相关,HIF-1α和COX-2的表达与放射性口腔黏膜炎的严重程度有关。更多还原

【Abstract】 Objective To investigate the mRNA and protein expressions of HIF-1 alpha and COX-2 in the rat tissues with radiation induced mucositis(ROM),and to analyze the correlation between the expression level of HIF-1 alpha and COX-2. Methods The rat model with ROM was established. The left buccal mucosa was excised as experimental tissue,whereas the right buccal mucosa was excised to serve as control tissue. The relative mRNA expressions of HIF-1 alpha and COX-2 were measured using the semi quantitative RT-PCR. The immunohistochemistry of Strept Avidin-Biotin-Complex Method (SABC Method) was employed to detect the distribution and intensity of HIF-1 alpha and COX-2 proteins. Results We successfully established a Sprague-Dawley rat model of ROM. The results of RT-PCR indicated that the left buccal mucosa expressed HIF-1 alpha and COX-2 mRNAs,while the right buccal mucosa showed negative expression of both genes. Immunohistochemical analysis demonstrated simultaneous expressions of HIF-1 alpha and COX-2 proteins in the left side mucosa. Conclusions The mucosa of ROM expresses HIF-1 alpha and COX-2 at mRNA and protein levels,which is correlated with the severity of ROM. A high correlation between HIF-1 alpha and COX-2 expressions has also been confirmed in this study.更多还原