【摘要】 目的:观察甘草酸二铵(DG)对大鼠脊髓缺血再灌注损伤后脊髓前角运动神经元Caspase-3表达的影响。方法:60只大鼠随机分为3组,每组20只。两组采用手术夹闭大鼠左右肾动脉之间的腹主动脉30min致脊髓缺血,然后松开再灌注,其中一组在缺血前10min舌下静脉注射DG20mg/kg(DG干预组),一组仅造成脊髓缺血损伤(损伤组);另一组打开腹腔后即关腹,不进行缺血再灌注(正常对照组)。分别于术后3、24、72、16 8h处死大鼠取腰段脊髓行免疫组化染色,观察脊髓前角Caspase-3光密度变化情况;72、168h处死动物前先采用Behrmann 5点评分法评定大鼠后肢功能。结果:损伤组与干预组72h时后肢功能评分分别为4.2±0.45分、5.8±1.1分;168h时分别为5±0分、6.2±1.3分,两组相同时间比较有显著性差异。Caspase-3表达位于脊髓前角运动神经元胞浆。术后3、24、72、168h,正常对照组前角运动神经元的平均光密度为0.13±0.04、0.15±0.06、0.13±0.06、0.15±0.06,损伤组为0.18±0.04、0.18±0.03、0.20±0.04、0.20±0.05,DG干预组为0.17±0.06、0.15±0.02、0.16±0.04、0.15±0.02,损伤组各时间点与对照比较均有显著性差异,DG干预组在24、72、168h与损伤组比较差异有显著性。结论:DG可下调凋亡信号转导通路中Caspase-3的表达水平,从而抑制脊髓前角神经元凋亡的发生。更多还原

【Abstract】 Objective:To investigate the effect of diammonium glycyrrhizinate(DG) on expression of caspase-3 in rat comuanterius medullae spinalis using ischemia reperfusion injury model.Method:60 rats were divided into blank group,control group and DG treated group,each group contains 20 rats.The protocol was established by intercepting rat abdominal aorta between right and left renal arter branches for 30min.The animals in DG treated group were injected DG 20mg/kg 10min via Vena sublingualis prior to ischemia start,the control group received nothing for ischemia-reperfusion injury,while the blank group received the procedure of open abdomen without introduction of ischemia-reperfusion injury.The expression of caspase-3 optical density in comuanterius medullae spinalis were measured at 3th、24th、72th、168th hour’ time-point respectively,and the socre of limb function at 72th、168th hour were evaluated using Behrmann method prior to testing the caspase-3 expression.Result:The mean score of limb function were 4.2±0.45 and 5±0 for blank group and DG intervention group at 72th hours time point,5.8±1.1 and 6.2±1.3 at 168th hour time pointrespectively,which had significant difference.Caspase-3 was expressed in cytolymph of motor neuron at 3th、24th、72th and 168th time point respectively.At 3th、24th、72th and 168th time point,the average level of caspase-3 optical density in blank group were 0.13±0.04、0.15±0.06、0.13±0.06、0.15±0.06,while 0.18±0.04、0.18±0.03、0.20±0.04、 0.20±0.05 in control group,and 0.17±0.06、0.15±0.03、0.16±0.04 in DG intervention group.There was statistical significance between groups at each time point.Conclusion:DG can down-regulate the level of caspase-3 in signal transduction pathway,which is useful in protecting the spinal cord in ischemia-reperfusion.更多还原